Title page for ETD etd-02012004-044143

Type of Document

Thesis

Author

Nicholas, Alexander Hubert

Author's Email Address

nicholas@psy.fsu.edi

URN

etd-02012004-044143

Title

Group I and II Metabotropic Glutamate Receptors are Necessary for the Activity-Dependent Maintenance of Ribosomal Integrity in Chick Auditory Neurons.

Degree

Master of Science

Department

Psychology, Department of

Advisory Committee

Advisor Name	Title
Richard Hyson	Committee Chair
Frank Johnson	Committee Member
Thomas Joiner	Committee Member

Keywords

Ribosome
Nucleus Magnocellularis
Cell Death
Deafferentation
Avian

Date of Defense

2004-01-23

Availability

unrestricted

Abstract

Elimination of eighth-nerve activity results in the death of 30% of the neurons in the chick cochlear nucleus, n. magnocellularis (NM). One early event in this cell death cascade is the disruption of ribosomes in NM neurons, which can be observed within one hour following deafferentation. These rapid changes in ribosomal integrity can be visualized using Y10b, a monoclonal antibody that recognizes ribosomal RNA. Previous studies using a brain slice preparation of the avian brain stem auditory system have shown that activation of metabotropic glutamate receptors (mGluRs) is necessary for the activity-dependent maintenance of ribosomal integrity. The purpose of the present study was to determine if group I and/or II mGluRs are necessary for this activity-dependent regulation. This was accomplished by selectively blocking group I or II receptors while unilaterally stimulating the auditory nerve in vitro. Y10b immunostaining served as the marker for ribosomal integrity. In normal media, unilateral stimulation of the auditory nerve resulted in darker Y10b labeling of NM neurons on the stimulated side of the slice. The group I antagonist (RS)-1-aminoindan-1,5-dicarboxylic acid (AIDA) and the group II antagonists LY341495 and (S)- -ethylglutamic acid (EGLU) all prevented the activity-dependent difference in immunolabeling. These data suggest that both group I and II mGluRs play vital roles in the activity-dependent maintenance of ribosomal integrity in NM.

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