Title page for ETD etd-05122008-111216

Type of Document

Thesis

Author

Liao, Tianqing

Author's Email Address

tqliao@gmail.com

URN

etd-05122008-111216

Title

Thermoresponsive Polyelectrolyte Multilayer Films As Culture Substrates for Human Mesenchymal Stem Cells

Degree

Master of Science

Department

Chemical Engineering, Department of

Advisory Committee

Advisor Name	Title
Joseph Schlenoff	Committee Member
Samuel Grant	Committee Member
Teng Ma	Committee Member

Keywords

Thermoresponsive
Stem Cells
Polyelectrolyte

Date of Defense

2008-04-14

Availability

unrestricted

Abstract

Human mesenchymal stem cells (hMSCs) are colony-forming unit fibroblasts (CFU-F) derived from adult bone marrow and have significant potential for many cell-based tissue-engineering applications. Their therapeutic potential, however, is restricted by the diminishing plasticity as they are expanded in culture. In this study, we investigated using N-isopropylacrylamide (NIPAM)-based thermoresponsive polyelectrolyte multilayer films as a culture substrate to support hMSC expansion without compromising their stem cell characteristics. The thermoresponsive surface is hydrophobic at culture temperature (37 °C) and hydrophilic at lower temperature (<4 °C), which enabling cell detachment without enzymatic treatment. The thermal responsive PEMU films were made via layer by layer adsorption of thermoresponsive polymers copolymerized with oppositely charged polyelectrolytes. Surface charges were shown to alter the ECM structure and subsequently regulate hMSC adhesion, proliferation, detachment, and colony forming ability. The positively charged thermal responsive surfaces improved cell adhesion and growth while preserving their CFU-F forming ability. These results demonstrate that PNIPAM-based thermal responsive surfaces are superior to the conventional culture method for hMSC in vitro expansion.

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