Type of Document Dissertation Author Jeffries-Grant, Kirsten URN etd-05122008-222634 Title Comparative Analysis of Human Milk Peptides: Gestational and Lactation Stage Effects Degree Doctor of Philosophy Department Chemistry and Biochemistry, Department of Advisory Committee
Advisor Name Title Randolph L Rill Committee Chair Kenneth Roux Committee Member Timothy Logan Committee Member William Cooper Committee Member Keywords
- Peptide Profile
- Human Milk
Date of Defense 2007-12-13 Availability unrestricted AbstractABSTRACT
In this study methods were developed for the analysis of endogenous human milk peptides with molecular weights < 6000 Da. Human milk peptides were purified by a one-step acid precipitation using 6% TCA (final concentration) and liquid-liquid extraction with 40:60 4-butanol: di-isopropyl ether (final concentration). The aqueous phase from organic extraction contained the peptides of interest and was desalted and concentrated using C18 SPE columns and C18 Ziptips™ for analysis by MALDI-TOF MS. The peptide profile was determined and compared for milks collected from mothers delivering preterm (weeks 1 and 2 of lactation) and full-term infants (weeks 1, 7, 12, and 16 of lactation). Overall more peptides were observed in preterm milks than in full-term milks. Masses corresponding to known and unknown human milk peptides were assigned to the peptide masses obtained by MALDI MS. Some of the assigned peptides were different forms of ghrelin, gastrin, and somatostatin. In addition to assigning peptides by mass, peptides were identified by collecting MS/MS data by nLC-FT-ICR-MS/MS and submission to a Mascot search of the human MSDB database. Peptides were identified corresponding to fragments of major milk proteins such as b-, k-, and áS1-caseins and bile salt-acid dependent lipase. One hundred percent sequence coverage was obtained for b-casein. Fragments pertaining to minor milk proteins were also identified such as secretory component and complement C4.
Filename Size Approximate Download Time (Hours:Minutes:Seconds)
28.8 Modem 56K Modem ISDN (64 Kb) ISDN (128 Kb) Higher-speed Access JeffriesGrantKDissertation.pdf 0.00 Kb < 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01