Type of Document	Thesis
Author	Kuricheti, Kalyan K
URN	etd-06102004-114908
Title	Application of Fluorescence Correlation Spectroscopy based Velocity Imaging In Microfluidics
Degree	Master of Science
Department	Chemical Engineering, Department of
Advisory Committee	Advisor Name Title Bruce R. Locke Committee Co-Chair Kenneth D Weston Committee Co-Chair Ravi Chella Committee Member
Keywords	Velocity Imaging Fluorescence Correlation Spectroscopy Microfluidics
Date of Defense	2004-05-24
Availability	unrestricted
Abstract Manipulation and transport of fluids in micro scale geometries in chemical and biological analysis is of great interest due to the inherent reduction in cost of fluid handling systems and reagents as well as the ease at which chemical analyses might be run in parallel for high throughput applications. While the flow of fluids at microscale is fundamentally simple to develop and analyze theoretically, experimental results have been inconsistent and contradictory. Experimental studies have also confirmed that there is a need for improved investigative tools for studying microflows. A number of methods for flow visualization applicable to micro-scale flow geometries have evolved over the last few years. Micro-Particle image velocimetry (µ-PIV) is the only commonly used method that has been demonstrated to have sub-micron spatial resolutions. In this thesis work, Fluorescence correlation spectroscopy imaging method was developed, characterized, optimized and applied to study the flow of fluid in PDMS-glass microfluidic devices. This is first application of FCS for velocity imaging applications. Using the method, velocities can be mapped in all three dimensions with excellent spatial resolutions. Another significant advantage of this method compared to other methods is the ability to use single molecules to image velocity fields as compared to nanometer-sized microspheres. Fluid flow in the Reynolds number range of 0.01 to 10 is investigated for any deviations from theoretical predictions. The effect of various parameters including the laser intensity, concentration of tracer particles, and acquisition times on the precision of the measurements was studied. A phenomenon that was previously misidentified as optical trapping effect was investigated. Our measurements provide conclusive evidence that this is infact an artifact caused by detector saturation.
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