Type of Document	Thesis
Author	Chase, Rose Ann
Author's Email Address	r_a_chase@yahoo.com
URN	etd-06142007-235804
Title	Initial Characterization of Two Alpha-Helical Membrane Proteins from Mycobacterium Tuberculosis
Degree	Master of Science
Department	Chemistry and Biochemistry, Department of
Advisory Committee	Advisor Name Title Timothy Cross Committee Chair Debra Ann Fadool Committee Member Marie Krafft Committee Member Timothy Logan Committee Member
Keywords	NMR Solution NMR Solid State NMR Maldi-tof Nhmfl
Date of Defense	2007-06-06
Availability	unrestricted
Abstract Rv3004 and Rv1861 are membrane proteins from Mycobacterium tuberculosis. Rv3004 is a 112 amino acid protein with one predicted transmembrane helix. Rv1861 is a 101 amino acid protein with three predicted transmembrane helices. Both proteins can be successfully labeled with 15N and expressed in high yields. Based on the NMR data, Rv3004 is a very stable protein that has very little solvent accessibility. Rv1861 is likely to be a high weight oligomer in its natural state. It is also very well structured and has two transmembrane helices oriented at a 37º tilt relative to the bilayer normal.
Files	Filename       Size       Approximate Download Time (Hours:Minutes:Seconds)     28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access    Thesis_submitted.pdf 960.92 Kb 00:04:26 00:02:17 00:02:00 00:01:00 00:00:05