Type of Document Thesis Author Elam, Marcus L Author's Email Address email@example.com URN etd-07252011-160657 Title The Effectiveness of Ferutinin on Osteoblastic Differentiation and Mineralization in MC3T3-E1 Cells Degree Master of Science Department Nutrition, Food, and Exercise Science, Department of Advisory Committee
Advisor Name Title Bahram H. Arjmandi Committee Chair Maria Spicer Committee Member Cathy W. Levenson University Representative Keywords
- MC3T3-E1 cells
- alkaline phosphatase
Date of Defense 2011-06-29 Availability restricted AbstractBackground: Osteoporosis is a serious condition in which bone of the skeleton deteriorates substantially, resulting from an imbalance of the bone remodeling process. Affecting more than 40 million Americans, osteoporosis leads to major fractures of the hip, spine. Although many forms of treatment are widely used, including anabolic and anti-resorptive agents, the vast majority of prescribed medications have minor to severe adverse effects. Thus, many sufferers of osteoporosis seek natural forms of treatment. Estrogen replacement therapy is effective in preventing and treating ovarian hormone-deficiency bone loss, yet the risks far exceed the benefits. Similar to soy isoflavonic compounds, which have been shown to exude beneficial effects on bone metabolism, ferutinin, a daucane extract of the giant fennel (Ferula communis) are believed to have estrogen-like activity.
Purpose: To elucidate the effects of ferutinin on osteoblastic differentiation and mineralization.
Methods: MC3T3-E1 preosteoblast-like cells were treated with ferutinin (10-12, 10-10, 10-8, 10-6, 10-5, 10-4 M), 17ß-estradiol (10-8 M), or no treatment (control). Cells cultured in an osteogenic medium (50 μg/ml ascorbic acid & 10 mM ß-glycerophosphate) contatining 5% charcoal-dextran treated FBS for up to nine, fifteen, or twenty-eight days for the assessment of the following: cell viability using an MTT assay, alkaline phosphatase activity measured colorimetrically, calcium nodule formation and mineralization using 40 mM alizarin red stain which was lifted and absorbance measured, and osteocalcin concentrations.
Results: Cell viability remained stable in cells treated with 10-12, 10-10, 10-8, and 10-6 M of ferutinin. Alkaline phosphatase activity was enhanced but not significantly (13.4% greater than control) in cells treated with 10-10 M ferutinin. Osteocalcin levels and the absorbance of retained stain ferutinin-treated cells were not significant.
Conclusions: Ferutinin at a dose of 10-10 M appeared to have a mild augmenting effect on osteoblastic differentiation; however, further experimentation with other in vitro and vivo models is needed to fully elucidate its effects.
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