Type of Document	Thesis
Author	Elam, Marcus L
Author's Email Address	mle09@my.fsu.edu
URN	etd-07252011-160657
Title	The Effectiveness of Ferutinin on Osteoblastic Differentiation and Mineralization in MC3T3-E1 Cells
Degree	Master of Science
Department	Nutrition, Food, and Exercise Science, Department of
Advisory Committee	Advisor Name Title Bahram H. Arjmandi Committee Chair Maria Spicer Committee Member Cathy W. Levenson University Representative
Keywords	Ferutinin MC3T3-E1 cells differentiation alkaline phosphatase
Date of Defense	2011-06-29
Availability	restricted
Abstract Background: Osteoporosis is a serious condition in which bone of the skeleton deteriorates substantially, resulting from an imbalance of the bone remodeling process. Affecting more than 40 million Americans, osteoporosis leads to major fractures of the hip, spine. Although many forms of treatment are widely used, including anabolic and anti-resorptive agents, the vast majority of prescribed medications have minor to severe adverse effects. Thus, many sufferers of osteoporosis seek natural forms of treatment. Estrogen replacement therapy is effective in preventing and treating ovarian hormone-deficiency bone loss, yet the risks far exceed the benefits. Similar to soy isoflavonic compounds, which have been shown to exude beneficial effects on bone metabolism, ferutinin, a daucane extract of the giant fennel (Ferula communis) are believed to have estrogen-like activity. Purpose: To elucidate the effects of ferutinin on osteoblastic differentiation and mineralization. Methods: MC3T3-E1 preosteoblast-like cells were treated with ferutinin (10-12, 10-10, 10-8, 10-6, 10-5, 10-4 M), 17ß-estradiol (10-8 M), or no treatment (control). Cells cultured in an osteogenic medium (50 μg/ml ascorbic acid & 10 mM ß-glycerophosphate) contatining 5% charcoal-dextran treated FBS for up to nine, fifteen, or twenty-eight days for the assessment of the following: cell viability using an MTT assay, alkaline phosphatase activity measured colorimetrically, calcium nodule formation and mineralization using 40 mM alizarin red stain which was lifted and absorbance measured, and osteocalcin concentrations. Results: Cell viability remained stable in cells treated with 10-12, 10-10, 10-8, and 10-6 M of ferutinin. Alkaline phosphatase activity was enhanced but not significantly (13.4% greater than control) in cells treated with 10-10 M ferutinin. Osteocalcin levels and the absorbance of retained stain ferutinin-treated cells were not significant. Conclusions: Ferutinin at a dose of 10-10 M appeared to have a mild augmenting effect on osteoblastic differentiation; however, further experimentation with other in vitro and vivo models is needed to fully elucidate its effects.
Files	Filename       Size       Approximate Download Time (Hours:Minutes:Seconds)     28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access  Elam_M_thesis_2011.pdf 907.05 Kb 00:04:11 00:02:09 00:01:53 00:00:56 00:00:04 indicates that a file or directory is accessible from the campus network only.