Title page for ETD etd-08052011-105001

Type of Document

Thesis

Author

Shirk, Katelan

Author's Email Address

kshirk@bio.fsu.edu

URN

etd-08052011-105001

Title

The Characterization of the Roles of Ipl1 and Cdc5 in Spindle Pole Body Duplication in Yeast Meiosis

Degree

Master of Science

Department

Biological Science, Department of

Advisory Committee

Advisor Name	Title
Bryant P. Chase	Committee Member
Hong-Guo Yu	Committee Member
Timothy Megraw	Committee Member
Yanchang Wang	Committee Member

Keywords

Yeast
Spindle Pole Body Duplication
Meiosis

Date of Defense

2011-06-30

Availability

unrestricted

Abstract

The centrosome is the microtubule organizing center (MTOC) in higher eukaryotes. During meiosis, proper duplication and separation of the centrosomes are necessary for accurate chromosome segregation and leads to the production of gametes containing half of the parental genome. During meiotic interphase I, centrosomes are duplicated when chromosomes replicate. A pair of centrosomes establish a bipolar microtubule spindle that facilitates the segregation of homologs during meiosis I. Centrosomes duplicate once more at interphase II, when DNA duplication is absent, and form two independent spindles for sister chromatid separation during meiosis II. The centrosome in yeast is called the spindle pole body (SPB). Here we show that the Aurora Kinase Ipl1, which protects sister chromatid cohesion, is also required for the maintenance of a tight association between duplicated sister SPBs, referred to here as SPB cohesion. Premature loss of cohesion leads to SPB over-duplication and the formation of multipolar spindles during meiosis II. The Polo-like kinase Cdc5 is a licensing factor for SPB duplication at interphase II and promotes SPB separation during meiosis II. Our data suggests Ipl1 and Cdc5 interact antagonistically at the SPB to maintain proper duplication and separation of SPBs during meiosis.

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