Type of Document	Dissertation
Author	Singh, Mani Prabha
URN	etd-08182010-134131
Title	Energy Transfer in Bio-molecules - Mechanism, Validity and Applicability of Nano-metal Surface Energy Transfer
Degree	Doctor of Philosophy
Department	Chemistry and Biochemistry, Department of
Advisory Committee	Advisor Name Title Prof. G.F. Strouse Committee Chair Prof. A.E. Steigman Committee Member Prof. T.M. Logan Committee Member Prof. P.B. Chase University Representative
Keywords	Gold nanoparticles DNA Metal nanoparticles Energy transfer
Date of Defense	2010-08-06
Availability	unrestricted
Abstract Metal nanoparticles are an important tool in energy transfer assays. The aim of this dissertation is to look deeply into the properties of small gold nanoparticles (Au NP) and how they can affect the experimental observables in energy transfer assays. Absorption, photoluminescence and time-resolved experiments were performed on Au NP-dye pairs separated by dsDNA spacers to deduce the distance dependent quenching behavior for 2 nm Au NP. A range of dyes were studied with different emission frequencies to set the energetic limits to the quenching behavior of Au NP. The effect of intraband and interband electrons on the quenching behavior of Au NP is different as the intraband quenching follows a 1/R^4 behavior while the interband quenching is largely unaffected with distance. The role of NP size is important as the intraband term is highly size dependent and also because of the enhancement effects caused by larger sizes of metal nanoparticles. The NSET tool is applied for accurately measuring the intercalation of an organic dye into a dsDNA helix as a function of the linker length and salt concentration.
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