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Type of Document Dissertation Author Wang, Guifang URN etd-11062006-135625 Title Cell and Tissue Culture Model of Airway Mucosa and its Applications Degree Doctor of Philosophy Department Chemical Engineering, Department of Advisory Committee
Advisor Name Title Cheng-jen Chen Committee Co-Chair Soonjo Kwon Committee Co-Chair Ewa Bienkiewicz Committee Member Henry Lee Committee Member Young-Bin Park Committee Member Keywords
- Chitosan
- Antedrug
- Epithelial Cell
- Inflammation
- Remodeling
- Steroids
Date of Defense 2006-07-31 Availability unrestricted Abstract CELL AND TISSUE CULTURE MODEL OF AIRWAY MUCOSA AND ITS APPLICATIONS
Name: Guifang Wang
Department: Chemical and Biomedical Engineering
Major Professor: Soonjo Kwon
Degree: Ph.D
Term degree awarded: Fall, 2006
Airway inflammation and remodeling are two key processes in chronic airway disease such as asthma. Inflammation in the airways is the major effect leading to narrowing of small airways and alveolar wall destruction in chronic obstructive pulmonary disease such as asthma. Airway remodeling is another important factor in airway disease. It is apparent that well-defined airway changes occur within the airway wall in disease condition.
Epithelial cells and fibroblast are two important cells involved in the inflammation and remodeling process. In our study, we established the cell culture model of epithelial cells and fibroblast cells and the co-culture model to study the airway inflammation and remodeling processes. We studied the modulatory effect of anti-inflammatory steroids antedrugs on cytokines in airway inflammations in cultured epithelial cells and the effects of water-soluble chitosan in pro-inflammatory cytokines and wound healing process. Co-culture modulated their effects. We identified the underlying mechanisms by which steroids and water-soluble chitosan modulate the effect on proinflammatory/inhibitory cytokines in lung cell(s). The differential response of proteins to anti-inflammatory steroidal antedrugs and water soluble chitosan was studied using a novel proteomic analysis 2D gel electrophoresis.
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