Type of Document	Thesis
Author	Kitchen, Jacqueline R.
Author's Email Address	jacquiekitchen@sunflower.com
URN	etd-11092006-150636
Title	Cloning and Expression of Notch ELRs 10-13 and Delta EBD
Degree	Master of Science
Department	Chemistry and Biochemistry, Department of
Advisory Committee	Advisor Name Title John Dorsey Committee Member Nancy Greenbaum Committee Member Timothy Logan Committee Member
Keywords	Protein Expression Cloning Delta Notch
Date of Defense	2006-11-08
Availability	unrestricted
Abstract Notch signaling plays an important role in tissue differentiation and remodeling and has been extensively studied. Much has been learned about the Notch signaling pathway and consequences of defects within the path, yet little is understood about the structure and biochemistry of the Notch receptor/Notch ligand interaction responsible for initiating the signaling events. Our goal is to investigate the biophysical interactions of Notch with its ligands Delta and Serrate. Here we describe the cloning and expression of Drosophila Notch ELRs 10-13 and Delta EBD, as well as initial isolation schemes for a Delta EBD-His construct.
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