Type of Document	Thesis
Author	Penagonda Srinivasa Rangachar, Vijay
Author's Email Address	vijay.penagonda@gmail.com
URN	etd-11132007-232037
Title	Impact of Lipopolysaccharide Extraction on Bacterial Transport
Degree	Master of Science
Department	Civil and Environmental Engineering, Department of
Advisory Committee	Advisor Name Title Gang Chen Committee Chair Amy Chan Hilton Committee Member Tarek Abichou Committee Member
Keywords	Lipopolysaccharide Extraction Mass Transfer Kinetic Adsorption Bacterial Transport Breakthrough Curve
Date of Defense	2007-10-26
Availability	unrestricted
Abstract Increasing water demands owing to the growth of urbanized populations have led to constraints on existing surface and groundwater supplies. Reclaimed wastewater thus has the potential to be used for agricultural irrigation. During the practice of reclaimed wastewater reuse, special care must be taken to monitor the fate and transport of the infectious agents or pathogenic organisms existing in the reclaimed wastewater since they may pass through the vadose zone and contaminate the groundwater. It is hypothesized lipopolysaccharide extraction would enhance bacterial interactions with the porous media, resulting maximum retention of the strains in the soil. Thus reclaimed wastewater can be recommended to go through relevant treatment process to remove bacterial lipopolysaccharide before it is practiced for agricultural irrigation. Lab cultured gram-negative bacterial strains, Escherichia coli, Pseudomonas aeruginosa and Pseudomonas fluorescens were used in the research. Lipopolysaccharide of these strains was extracted and column experiments were then conducted for both treated and untreated bacterial strains with silica sand serving as the porous media. Transport of these strains was described by the equilibrium-kinetic two-region solute transport model by fitting the experimentally obtained bacterial breakthrough data using CXTFIT 2.1. Bacteria were retained in the media during transport for all the three strains with or without lipopolysaccharide extraction. Observations after lipopolysaccharide extraction showed greater retention for all the strains as compared to the original strains, indicating enhanced bacterial interaction within the media. Electrostatic interations between bacterial strains and porous media were calculated based on the measured zeta potential values of the strains and the media. It was found that bacterial deposition coefficient linearly decreased with the increase of the electrostatic interactions. After lipopolysaccharide extraction, bacterial electrostatic interactions with the porous media
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