Type of Document	Thesis
Author	Larion, Mioara
Author's Email Address	mlarion@chem.fsu.edu
URN	etd-11142005-112607
Title	Sensitivity of ST-EPR to the Rate of Motion at X and W-Band
Degree	Master of Science
Department	Chemistry and Biochemistry, Department of
Advisory Committee	Advisor Name Title Timothy Logan Committee Chair Ken Weston Committee Member Michael Blaber Committee Member Piotr. G. Fajer Committee Member
Keywords	X-band W-band Hemoglobin nitroxide spin label Molecular Dynamics Saturation Transfer Electron Paramagnetic Resonanc
Date of Defense	2005-08-18
Availability	unrestricted
Abstract ST-EPR, developed by Hyde, Thomas and Dalton in 1972 is a nonlinear EPR method that measures the transfer of saturation throughout a spectrum by rotational molecular motions in the microseconds to millisecond time range (conventional EPR not sensitive on this timescale). Sensitivity of ST-EPR was reported for 9 GHz (Hyde et al., 1980) and for 35 GHz (Johnson et al., 1981; Johnson et al., 1982) suggesting the advantages of increasing the Larmor frequency. The present study investigates the sensitivity of ST-EPR at higher microwave frequencies (95 GHz) and compares it to lower microwave frequency (9 GHz). Using a model system of spin labeled hemoglobin tumbling in the water/glycerol mixtures of varying viscosity; we show a significant increase in ST-EPR sensitivity at W-band. This advantage of high frequency ST-EPR is overshadowed by the increased instrumental noise levels and phase instability in the commercial Bruker W-band spectrometer.
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